HEK-293 cells were subjected to SDS PAGE followed by western blot with 12548-1-AP (CPVL Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12548-1-AP (CPVL Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 12548-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 12548-1-AP (CPVL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
human heart tissue were subjected to SDS PAGE followed by western blot with 12548-1-AP (CPVL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 12548-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 12548-1-AP (CPVL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
human liver tissue were subjected to SDS PAGE followed by western blot with 12548-1-AP (CPVL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 12548-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 12548-1-AP (CPVL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
HepG2 cells were subjected to SDS PAGE followed by western blot with 12548-1-AP (CPVL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 12548-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 12548-1-AP (CPVL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
human kidney tissue were subjected to SDS PAGE followed by western blot with 12548-1-AP (CPVL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 12548-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12548-1-AP (CPVL antibody) at dilution of 1:2000 incubated at 4 degree celsius over night.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12548-1-AP (CPVL antibody) at dilution of 1:2000 incubated at 4 degree celsius over night.
IHC staining of human liver cancer using 12548-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer using 12548-1-AP (CPVL antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12548-1-AP (CPVL Antibody) at dilution of 1:200 (under 40x lens).
IF Staining of HepG2 using 12548-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 12548-1-AP (CPVL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 12548-1-AP (CPVL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
FC experiment of HepG2 using 12548-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CPVL Polyclonal antibody (12548-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CPVL Polyclonal antibody (12548-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
HEK-293 cells, HepG2 cells, human heart tissue, human kidney tissue, human liver tissue
Positive IP detected in
HEK-293 cells
Positive IHC detected in
human liver cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Positive FC (Intra) detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:4000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Dissecting gastric cancer heterogeneity and exploring therapeutic strategies using bulk and single-cell transcriptomic analysis and experimental validation of tumor microenvironment and metabolic interplay
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12548-1-AP (CPVL Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 12548-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 12548-1-AP (CPVL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 12548-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 12548-1-AP (CPVL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 12548-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 12548-1-AP (CPVL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 12548-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 12548-1-AP (CPVL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human liver cancer using 12548-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer using 12548-1-AP (CPVL antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver cancer using 12548-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer using 12548-1-AP (CPVL antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human liver using 12548-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12548-1-AP (CPVL Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver using 12548-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12548-1-AP (CPVL Antibody) at dilution of 1:200 (under 40x lens).
IP Figures
IP experiment of HEK-293 using 12548-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12548-1-AP (CPVL antibody) at dilution of 1:2000 incubated at 4 degree celsius over night.
IF/ICC Figures
IF Staining of HepG2 using 12548-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 12548-1-AP (CPVL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
FC (INTRA) Figures
FC experiment of HepG2 using 12548-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CPVL Polyclonal antibody (12548-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs.
We have selected the top cited antibodies from these vendors for you to compare.
Proteintech
CPVL Polyclonal antibody
Catalog Number
12548-1-AP
Citations
1
Dilutions
WB : 1:1000-1:4000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:50-1:500 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
