CPVL Polyclonal antibody

CPVL Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12548-1-AP

Synonyms

VCP like protein, UNQ197/PRO223, PSEC0124, hVLP, EC:3.4.16.-

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Validation Data Gallery

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  WB analysis of HEK-293 using 12548-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12548-1-AP (CPVL Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 12548-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12548-1-AP (CPVL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 12548-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12548-1-AP (CPVL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12548-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12548-1-AP (CPVL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 12548-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12548-1-AP (CPVL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 12548-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12548-1-AP (CPVL antibody) at dilution of 1:2000 incubated at 4 degree celsius over night.

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  IHC staining of human liver cancer using 12548-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 12548-1-AP (CPVL antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver cancer using 12548-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 12548-1-AP (CPVL antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 12548-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12548-1-AP (CPVL Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 12548-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12548-1-AP (CPVL Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 12548-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 12548-1-AP (CPVL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 12548-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CPVL Polyclonal antibody (12548-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, human heart tissue, human kidney tissue, human liver tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human liver cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

12548-1-AP targets CPVL in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CPVL fusion protein Ag3398
• Full Name: carboxypeptidase, vitellogenic-like
• Calculated Molecular Weight: 476 aa, 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC016838
• Gene Symbol: CPVL
• Gene ID (NCBI): 54504
• RRID: AB_2084865
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Front Pharmacol; Dissecting gastric cancer heterogeneity and exploring therapeutic strategies using bulk and single-cell transcriptomic analysis and experimental validation of tumor microenvironment and metabolic interplay; Authors - XianTao Lin