CPSF6 Polyclonal antibody

• Featured Product
• KD/KO Validated

CPSF6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

9

Reviews (1)

Cat no : 15489-1-AP

Synonyms

CPSF 68 kDa subunit, Cleavage factor Im complex 68 kDa subunit, Cleavage and polyadenylation specificity factor subunit 6, Cleavage and polyadenylation specificity factor 68 kDa subunit, CFIm68

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Validation Data Gallery

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  WB analysis of HeLa using 15489-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15489-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15489-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15489-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15489-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15489-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15489-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 15489-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 15489-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15489-1-AP

  IP result of anti-CPSF6 (IP:15489-1-AP, 4ug; Detection:15489-1-AP 1:300) with HeLa cells lysate 3200ug.

• 

  IHC staining of human kidney using 15489-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15489-1-AP (CPSF6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15489-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15489-1-AP (CPSF6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 15489-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15489-1-AP (CPSF6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 15489-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15489-1-AP (CPSF6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 15489-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15489-1-AP (CPSF6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 15489-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15489-1-AP (CPSF6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of SW480 using 15489-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SW480 cells using CPSF6 antibody (15489-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells, PC-3 cells, MDA-MB-231 cells, SW480 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver tissue, human heart tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SW480 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 9 publications below

Product Information

15489-1-AP targets CPSF6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CPSF6 fusion protein Ag7852
• Full Name: cleavage and polyadenylation specific factor 6, 68kDa
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 55-68 kDa
• GenBank Accession Number: BC000714
• Gene Symbol: CPSF6
• Gene ID (NCBI): 11052
• RRID: AB_10694140
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The binding of Cleavage factor Im (CFIM), also known as CPSF6, to the pre-mRNA is one of the earliest steps in the assembly of the cleavage and polyadenylation machinery and facilitates the recruitment of other processing factors. CFIM is required for the first step in pre-mRNA 3′-end processing and can be reconstituted in vitro from its heterologously expressed 25- and 68-kDa subunits. It involved in RNA binding, protein-protein interactions, and subcellular localization [PMID:15169763]. In addition, it is a pre-mRNA processing protein that dynamically shuttles between the nucleus and the cytoplasm and contains a C-terminal nuclear-targeting arginine/serine-rich (RS-) domain of the type bound by TNPO3[PMID:15169763,19864460].

Publications

Species	Application	Title
human	WB	Mol Cell; A Quantitative Genetic Interaction Map of HIV Infection.; Authors - David E Gordon
human	WB	PLoS Pathog; In Vivo Functions of CPSF6 for HIV-1 as Revealed by HIV-1 Capsid Evolution in HLA-B27-Positive Subjects.; Authors - Matthew S Henning
human	WB	Elife; Nuclear pore heterogeneity influences HIV-1 infection and the antiviral activity of MX2.; Authors - Melissa Kane
human	WB	J Virol; Cell type-dependent escape of capsid inhibitors by simian immunodeficiency virus SIVcpz.; Authors - Augustin Penda Twizerimana
human	WB	iScience; Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.; Authors - Mio Iwasaki
human	WB	J Virol; Roles of capsid-interacting host factors in multimodal inhibition of HIV-1 by PF74.; Authors - Akatsuki Saito ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tatyana (Verified Customer) (03-11-2023)

The signal is not very bright but specific (siRNA knockdown verified). Predominantly nuclear, as expected. 4% PFA fixation followed by methanol permeabilization; antibody prepared in 5% goat serum in PBST; overnight incubation (no blocking step).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human, SH-SY5Y cells