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- KD/KO Validated
CPSF3 Polyclonal antibody
CPSF3 Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IP, ELISA
Conjugate
Unconjugated
Cat no : 11609-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HepG2 cells, human brain tissue, human skin tissue |
Positive IP detected in | HEK-293 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 5 publications below |
Product Information
11609-1-AP targets CPSF3 in WB, IP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CPSF3 fusion protein Ag2173 |
Full Name | cleavage and polyadenylation specific factor 3, 73kDa |
Calculated Molecular Weight | 684 aa, 77 kDa |
Observed Molecular Weight | 77 kDa |
GenBank Accession Number | BC020211 |
Gene Symbol | CPSF3 |
Gene ID (NCBI) | 51692 |
RRID | AB_2292188 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for CPSF3 antibody 11609-1-AP | Download protocol |
IP protocol for CPSF3 antibody 11609-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Exp Clin Cancer Res LncRNA CASC9 interacts with CPSF3 to regulate TGF-β signaling in colorectal cancer.
| ||
Cancers (Basel) CPSF3 Promotes Pre-mRNA Splicing and Prevents CircRNA Cyclization in Hepatocellular Carcinoma | ||
Cell Rep Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome. | ||
Cell Mol Life Sci Single-cell multi-omics profiling reveals key regulatory mechanisms that poise germinal vesicle oocytes for maturation in pigs | ||
Cell Chem Biol Anticancer benzoxaboroles block pre-mRNA processing by directly inhibiting CPSF3 |