CPPED1 Polyclonal antibody

CPPED1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20628-1-AP

Synonyms

CPPED1, CSTP1

---

Validation Data Gallery

• 

  WB analysis of SMMC-7721 using 20628-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 20628-1-AP (CPPED1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 20628-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20628-1-AP (CPPED1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 20628-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20628-1-AP (CPPED1 Antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: SMMC-7721 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20628-1-AP is CPPED1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CPPED1 fusion protein Ag14572
• Full Name: calcineurin-like phosphoesterase domain containing 1
• Calculated Molecular Weight: 314 aa, 36 kDa
• Observed Molecular Weight: 28-35 kDa
• GenBank Accession Number: BC006289
• Gene Symbol: CPPED1
• Gene ID (NCBI): 55313
• RRID: AB_2878711
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CPPED1, also named as CSTP1, plays an inhibitory role in glucose uptake by adipocytes. It has some isoforms with MW 20 kDa and 28-35 kDa.