CPNE6 Polyclonal antibody

CPNE6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 13782-1-AP

Synonyms

Copine 6, Copine VI, copine VI (neuronal), CPNE6, N COPINE, Neuronal copine

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Validation Data Gallery

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  WB analysis using 13782-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13782-1-AP (CPNE6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat dorsal root ganglion using 13782-1-AP

  Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue slide using 13782-1-AP (CPNE6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat dorsal root ganglion using 13782-1-AP

  Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue slide using 13782-1-AP (CPNE6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: rat dorsal root ganglion tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

13782-1-AP targets CPNE6 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CPNE6 fusion protein Ag4789
• Full Name: copine VI (neuronal)
• Calculated Molecular Weight: 557 aa, 62 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC018046
• Gene Symbol: CPNE6
• Gene ID (NCBI): 9362
• RRID: AB_2292172
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Copines are a family of evolutionarily conserved calcium-dependent phospholipid-binding proteins (PMID: 9430674). They contain two Ca(2+)- and phospholipid-binding domains known as C2 domains. Copines are potentially involved in regulating membrane trafficking and in protein-protein interactions.

Publications

Species	Application	Title
mouse	WB	Front Mol Neurosci; Integrated proteomics and metabolomics analysis of D-pinitol function during hippocampal damage in streptozocin-induced aging-accelerated mice; Authors - Xiaoxia Li
human, rat	WB,IF	J Neurol Sci; Increased expression of copine VI in patients with refractory epilepsy and a rat model.; Authors - Binglin Zhu
human,rat	IP,WB,CoIP	Cell Rep; Copine-6 is a Ca2+ sensor for activity-induced AMPA receptor exocytosis; Authors - Jing Zhi Anson Tan

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