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CPNE2 Polyclonal antibody

CPNE2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17664-1-AP

Synonyms

Copine 2, copine II, COPN2, CPN2, CPNE2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using CPNE2 Polyclonal antibody (17664-1-AP)

    WB analysis of mouse brain using 17664-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17664-1-AP (CPNE2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CPNE2 Polyclonal antibody (17664-1-AP)

    IHC staining of mouse brain using 17664-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17664-1-AP (CPNE2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CPNE2 Polyclonal antibody (17664-1-AP)

    IHC staining of mouse brain using 17664-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17664-1-AP (CPNE2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17664-1-AP targets CPNE2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CPNE2 fusion protein Ag11946
Full Name copine II
Calculated Molecular Weight 548 aa, 61 kDa
Observed Molecular Weight61 kDa
GenBank Accession NumberBC053658
Gene Symbol CPNE2
Gene ID (NCBI) 221184
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Copines are a family of evolutionarily conserved calcium-dependent phospholipid-binding proteins (PMID: 9430674). They contain two Ca(2+)- and phospholipid-binding domains known as C2 domains. Copines are potentially involved in regulating membrane trafficking and in protein-protein interactions.

Protocols

Product Specific Protocols
WB protocol for CPNE2 antibody 17664-1-APDownload protocol
IHC protocol for CPNE2 antibody 17664-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols