CPN1 Polyclonal antibody

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  WB analysis using 31940-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31940-1-AP (CPN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

CPN1 Polyclonal Antibody for WB, ELISA

Cat No. 31940-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Kininase-1, Kininase 1, EC:3.4.17.3, CPN, Arginine carboxypeptidase

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Tested Applications

• Positive WB detected in: human plasma

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31940-1-AP targets CPN1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CPN1 fusion protein Ag34806
• Full Name: carboxypeptidase N, polypeptide 1
• Calculated Molecular Weight: 458 aa, 52 kDa
• Observed Molecular Weight: 40~50 kDa
• GenBank Accession Number: BC027897
• Gene Symbol: CPN1
• Gene ID (NCBI): 1369
• RRID: AB_3670152
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P15169
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human carboxypeptidase N (CPN), a member of the CPN/E subfamily of "regulatory" metallo-carboxypeptidases, is an extracellular glycoprotein synthesized in the liver and secreted into the blood, where it controls the activity of vasoactive peptide hormones, growth factors and cytokines by specifically removing C-terminal basic residues. Normally, CPN circulates in blood plasma as a hetero-tetramer consisting of two 83 kDa (CPN2) domains each flanked by a 48 to 55 kDa catalytic (CPN1) domain. The C terminal region of the CPN1 subunit differs from all other family members in that it contains numerous basic residues. Proteolysis at these sites apparently happens either constitutively in the blood or during processing and secretion from the liver.