CPN1 Polyclonal antibody, PBS Only

CPN1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13385-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 13385-1-AP (same clone as 13385-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13385-1-AP (CPN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13385-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 13385-1-AP (same clone as 13385-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 13385-1-AP (CPN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13385-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 13385-1-AP (same clone as 13385-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13385-1-AP (CPN1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13385-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 13385-1-AP (same clone as 13385-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13385-1-AP (CPN1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13385-1-PBS in a different storage buffer formulation.

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  IF Staining of COLO 320 using 13385-1-AP (same clone as 13385-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed COLO 320 cells using CPN1 antibody (13385-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 13385-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 13385-1-AP (same clone as 13385-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CPN1 antibody (13385-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 13385-1-PBS in a different storage buffer formulation.

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Product Information

13385-1-PBS targets CPN1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CPN1 fusion protein Ag4035
• Full Name: carboxypeptidase N, polypeptide 1
• Calculated Molecular Weight: 458 aa, 52 kDa
• Observed Molecular Weight: 40~50 kDa
• GenBank Accession Number: BC027897
• Gene Symbol: CPN1
• Gene ID (NCBI): 1369
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Human carboxypeptidase N (CPN), a member of the CPN/E subfamily of "regulatory" metallo-carboxypeptidases, is an extracellular glycoprotein synthesized in the liver and secreted into the blood, where it controls the activity of vasoactive peptide hormones, growth factors and cytokines by specifically removing C-terminal basic residues. Normally, CPN circulates in blood plasma as a hetero-tetramer consisting of two 83 kDa (CPN2) domains each flanked by a 48 to 55 kDa catalytic (CPN1) domain. The C terminal region of the CPN1 subunit differs from all other family members in that it contains numerous basic residues. Proteolysis at these sites apparently happens either constitutively in the blood or during processing and secretion from the liver.