Carboxypeptidase B2 Polyclonal antibody

Carboxypeptidase B2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 10672-1-AP

Synonyms

Carboxypeptidase B2, carboxypeptidase B2 (plasma), Carboxypeptidase U, CPB2, CPU, PCPB, Plasma carboxypeptidase B, TAFI

---

Validation Data Gallery

• 

  WB analysis of mouse liver using 10672-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10672-1-AP (Carboxypeptidase B2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 10672-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10672-1-AP (Carboxypeptidase B2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10672-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10672-1-AP (Carboxypeptidase B2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 10672-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10672-1-AP (Carboxypeptidase B2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IHC detected in: human colon cancer tissue, human liver tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

10672-1-AP targets Carboxypeptidase B2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Carboxypeptidase B2 fusion protein Ag1110
• Full Name: carboxypeptidase B2 (plasma)
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC007057
• Gene Symbol: Carboxypeptidase B2
• Gene ID (NCBI): 1361
• RRID: AB_2086000
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CPB2(Carboxypeptidase B2) belongs to the peptidase M14 family. The CPB2 gene encoding TAFI is a candidate gene for blood pressure. Circulating CPB2 and platelets may potentially contribute to regulating the bioactivity of leukocyte chemoattractant chemerin (RARRES2), and further extend the molecular link between blood coagulation/fibrinolysis and CMKLR1-mediated immune responses. This protein has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	WB	FEMS Immunol Med Microbiol; Secreted Bacillus anthracis proteases target the host fibrinolytic system.; Authors - Chung Myung-Chul MC
mouse	WB	Neurochem Res; Serum Proteomics Identified TAFI as a Potential Molecule Facilitating the Migration of Peripheral Monocytes to Damaged White Matter During Chronic Cerebral Hypoperfusion; Authors - Yuhan Wang