CPA4 Polyclonal antibody

CPA4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 26824-1-AP

Synonyms

Carboxypeptidase A3, carboxypeptidase A4, CPA3, CPA4

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Validation Data Gallery

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  WB analysis of mouse heart using 26824-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 26824-1-AP (CPA4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 26824-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 26824-1-AP (CPA4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 26824-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 26824-1-AP (CPA4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 26824-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 26824-1-AP (CPA4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 26824-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26824-1-AP (CPA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 26824-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26824-1-AP (CPA4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 26824-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26824-1-AP (CPA4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse heart tissue, SMMC-7721 cells, mouse kidney tissue, A549 cells, HepG2 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 2 publications below

Product Information

26824-1-AP targets CPA4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CPA4 fusion protein Ag25327
• Full Name: carboxypeptidase A4
• Calculated Molecular Weight: 421 aa, 47 kDa
• Observed Molecular Weight: 44 kDa, 150 kDa
• GenBank Accession Number: BC052289
• Gene Symbol: CPA4
• Gene ID (NCBI): 51200
• RRID: AB_2880648
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Carboxypeptidase A4 (CPA4), a member of the metallocarboxypeptidase family, is a zinc-containing exopeptidase that catalyzes the release of carboxy-terminal amino acids. CPA4 was previously shown to play a crucial role in cell growth and differentiation by modulating or inactivating peptide hormone activity (PMID: 27904708). CPA4 expression is increased in multiple cancer tissues, including tissues from patients with pancreatic cancer, gastric cancer, esophageal squamous cell carcinoma, and lung cancer, and may serve as a potential diagnostic and prognostic marker (PMID: 31397502, 32922037).

Publications

Species	Application	Title
human	WB	Sci Signal; Hypoxic cancer-associated fibroblasts increase NCBP2-AS2/HIAR to promote endothelial sprouting through enhanced VEGF signaling.; Authors - Fernanda G Kugeratski
human	WB,IHC	Acta Biochim Biophys Sin (Shanghai); Angiopoietin-1 promotes triple-negative breast cancer cell proliferation by upregulating carboxypeptidase A4; Authors - Xue Liu
human,mouse	WB	Cell Death Dis; Carboxypeptidase A4 negatively regulates HGS-ETR1/2-induced pyroptosis by forming a positive feedback loop with the AKT signalling pathway; Authors - Luoling Wang
human	IHC	J Cell Mol Med; CPA4 as a biomarker promotes the proliferation, migration and metastasis of clear cell renal cell carcinoma cells; Authors - Kongjia Wang