Carboxypeptidase A2 Polyclonal antibody

Carboxypeptidase A2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15626-1-AP

Synonyms

Carboxypeptidase A2, CPA2

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Validation Data Gallery

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  WB analysis of mouse pancreas using 15626-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15626-1-AP (Carboxypeptidase A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 15626-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15626-1-AP (Carboxypeptidase A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 15626-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 15626-1-AP (Carboxypeptidase A2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas using 15626-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 15626-1-AP (Carboxypeptidase A2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human pancreas using 15626-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 15626-1-AP (Carboxypeptidase A2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas using 15626-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 15626-1-AP (Carboxypeptidase A2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human pancreas using 15626-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 15626-1-AP (Carboxypeptidase A2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas using 15626-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 15626-1-AP (Carboxypeptidase A2 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse pancreas tissue
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

15626-1-AP targets Carboxypeptidase A2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Carboxypeptidase A2 fusion protein Ag8080
• Full Name: carboxypeptidase A2 (pancreatic)
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC014571
• Gene Symbol: Carboxypeptidase A2
• Gene ID (NCBI): 1358
• RRID: AB_2276735
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Transl Oncol; Label-Free Quantitative Proteomics Unravels Carboxypeptidases as the Novel Biomarker in Pancreatic Ductal Adenocarcinoma.; Authors - Yang Song