CPA1 Monoclonal antibody

CPA1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2H2E8

Cat no : 68826-1-Ig

Synonyms

Carboxypeptidase A1, EC:3.4.17.1, CPA, 2H2E8

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Validation Data Gallery

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  WB analysis using 68826-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68826-1-Ig (CPA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: pig pancreas tissue, rat pancreas tissue, mouse pancreas tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68826-1-Ig targets CPA1 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CPA1 fusion protein Ag8579
• Full Name: carboxypeptidase A1 (pancreatic)
• Calculated Molecular Weight: 419 aa, 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC005279
• Gene Symbol: Carboxypeptidase A1
• Gene ID (NCBI): 1357
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CPA1(Carboxypeptidase A1) is also named as CPA and belongs to the the peptidase M14 family. CPA1 is one of the genes whose expression in the pancreas and the protein which it encodes is synthesized as an inactive precursor, proCPA1, which is processed to the active enzyme by the proteolytic removal of the 95-amino acid N-terminal prodomain. The mature CPA1 is not secreted and is trapped and degraded intracellularly(PMID:8639628). This antibody is speicific to CPA1.