COXIV Monoclonal antibody

COXIV Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (2)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

8

CloneNo.

3C7D2

Cat no : 60251-1-Ig

Synonyms

Cell and organelle markers, COX IV 1, COX4, COX4I1, COXIV, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit 4 isoform 1 mitochondrial, Mitochondria Marker, Mitochondrial Marker, Mitochondrion Marker

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Validation Data Gallery

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  IHC staining of human prostate cancer using 60251-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 60251-1-Ig(COXIV antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human prostate cancer using 60251-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 60251-1-Ig(COXIV antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human pancreas using 60251-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas using 60251-1-Ig(COXIV antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas using 60251-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas using 60251-1-Ig(COXIV antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human pancreas using 60251-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas using 60251-1-Ig(COXIV antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 60251-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas using 60251-1-Ig(COXIV antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human heart using 60251-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 60251-1-Ig(COXIV antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 60251-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 60251-1-Ig(COXIV antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 60251-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 60251-1-Ig(COXIV antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 60251-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 60251-1-Ig(COXIV antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 60251-1-Ig

  Immunofluorescent analysis of HeLa cells using 60251-1-Ig (COXIV antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

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Published Applications

• WB: See 5 publications below
• IF: See 5 publications below

Product Information

60251-1-Ig targets COXIV in WB, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: COXIV fusion protein Ag20551
• Full Name: cytochrome c oxidase subunit IV isoform 1
• Calculated Molecular Weight: 19.6 kDa
• Observed Molecular Weight: 17-18 kDa
• GenBank Accession Number: BC021236
• Gene Symbol: COX IV
• Gene ID (NCBI): 1327
• RRID: AB_2881372
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. COX4I1 is commonly used as a loading control. This antibody is specific to COX4I1 and do not cross reacts with COX4I2.

Publications

Species	Application	Title
human	WB	Autophagy; The nucleoprotein of influenza A virus inhibits the innate immune response by inducing mitophagy; Authors - Bo Zhang
human	IF	Hypertension; Upregulation of Piezo1 (Piezo Type Mechanosensitive Ion Channel Component 1) Enhances the Intracellular Free Calcium in Pulmonary Arterial Smooth Muscle Cells From Idiopathic Pulmonary Arterial Hypertension Patients.; Authors - Jing Liao
mouse	IF	Mol Metab; Disruption of STIM1-mediated Ca2+ sensing and energy metabolism in adult skeletal muscle compromises exercise tolerance, proteostasis and lean mass.; Authors - Rebecca J Wilson
mouse	IF	Mol Cell Biol; Protein Kinase A/CREB Signaling Prevents Adriamycin-Induced Podocyte Apoptosis via Upregulation of Mitochondrial Respiratory Chain Complexes.; Authors - Kewei Xie
rat	WB,IF	Eur J Histochem; Transcription factor nuclear factor erythroid 2 p45-related factor 2 (NRF2) ameliorates sepsis-associated acute kidney injury by maintaining mitochondrial homeostasis and improving the mitochondrial function.; Authors - Zhijiang Chen
rat	WB	Cells; Parkin-Dependent Mitophagy is Required for the Inhibition of ATF4 on NLRP3 Inflammasome Activation in Cerebral Ischemia-Reperfusion Injury in Rats.; Authors - Qi He