COX8A Polyclonal antibody

COX8A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 15368-1-AP

Synonyms

COX, COX8, COX8 2, COX8A, COX8L, VIII, VIII L

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Validation Data Gallery

• 

  WB analysis of mouse skeletal muscle using 15368-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15368-1-AP (COX8A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 15368-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15368-1-AP (COX8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 15368-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15368-1-AP (COX8A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 15368-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15368-1-AP (COX8A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of rat heart using 15368-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 15368-1-AP (COX8A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 15368-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 15368-1-AP (COX8A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 15368-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15368-1-AP (COX8A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 15368-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15368-1-AP (COX8A antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, human skeletal muscle tissue
• Positive IHC detected in: human heart tissue, human hepatocirrhosis tissue, rat heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

15368-1-AP targets COX8A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COX8A fusion protein Ag2885
• Full Name: cytochrome c oxidase subunit 8A (ubiquitous)
• Calculated Molecular Weight: 8 kDa
• Observed Molecular Weight: 8 kDa
• GenBank Accession Number: BC063025
• Gene Symbol: COX8A
• Gene ID (NCBI): 1351
• RRID: AB_10697832
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COX8A(Cytochrome c oxidase subunit 8A) is also named as COX8, COX8L and belongs to the cytochrome c oxidase VIII family. This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. The gene encodes a full length protein of 8 kDa with a transit peptide of 25 amino acid.

Publications

Species	Application	Title
human	WB	Cell Death Dis; O-GlcNAcylation on Rab3A attenuates its effects on mitochondrial oxidative phosphorylation and metastasis in hepatocellular carcinoma.; Authors - Weicheng Wu
human	IHC	J Proteomics; MALDI-MS tissue imaging identification of biliverdin reductase B overexpression in prostate cancer.; Authors - Pallua Johannes Dominikus JD
mouse	WB	Nat Commun; A FRET-based respirasome assembly screen identifies spleen tyrosine kinase as a target to improve muscle mitochondrial respiration and exercise performance in mice; Authors - Ami Kobayashi
rat	WB	Basic Res Cardiol; Comparison of the stage-dependent mitochondrial changes in response to pressure overload between the diseased right and left ventricle in the rat; Authors - Ling Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Svitlana (Verified Customer) (03-01-2019)

HEK293t transfected with Cox8-eGFP, 48 hours post-transfection cells collected for mitochondria isolation. 10 ug mitochondrial lysate (in RIPA buffer) was loaded per line.Incubation with primary anti-Cox8 antibody overnight at 4C.Image: 1st line protein ladder, 2nd line no-transfection sample, 3rd line Cox8-eGFP transfected sample.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293t

COX8A Antibody Western Blot, validation (1:1000 dilution) in HEK293t (Cat no:15368-1-AP)