COX6B2 Polyclonal antibody

COX6B2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11437-1-AP

Synonyms

Cancer/testis antigen 59, COX VIb 2, COX6B2, COXVIB2, CT59

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Validation Data Gallery

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  WB analysis using 11437-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11437-1-AP (COX6B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 11437-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11437-1-AP (COX6B2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 11437-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11437-1-AP (COX6B2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

11437-1-AP targets COX6B2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COX6B2 fusion protein Ag1983
• Full Name: cytochrome c oxidase subunit VIb polypeptide 2 (testis)
• Calculated Molecular Weight: 88 aa, 11 kDa
• Observed Molecular Weight: 11 kDa
• GenBank Accession Number: BC064548
• Gene Symbol: COX6B2
• Gene ID (NCBI): 125965
• RRID: AB_10859092
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COX6B2(Cytochrome c oxidase subunit 6B2) encodes the testis-specific cytochrome C oxidase subunit VIb. It has been shown to be expressed in a subset of lung and breast tumors at a level >1% of the testicular level of expression(PMID: 24491090). It belongs to the cytochrome c oxidase subunit 6B family.

Publications

Species	Application	Title
human	WB	J Biol Chem; PMID: 20870728; Authors - Oliva Claudia R CR