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  • KD/KO Validated

COX6A1 Polyclonal antibody

COX6A1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11460-1-AP

Synonyms

Cytochrome c oxidase subunit VIA-liver, Cytochrome c oxidase polypeptide VIa-liver, COX6AL, COX VIa-L, COX VIa L



Tested Applications

Positive WB detected inhuman brain tissue, NIH/3T3 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11460-1-AP targets COX6A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COX6A1 fusion protein Ag2006
Full Name cytochrome c oxidase subunit VIa polypeptide 1
Calculated Molecular Weight 85 aa, 9 kDa
Observed Molecular Weight 9.5 kDa
GenBank Accession NumberBC007723
Gene Symbol COX6A1
Gene ID (NCBI) 1337
RRIDAB_2085445
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COX6A1(Cytochrome c oxidase subunit 6A1, mitochondrial) is also named as COX6AL and belongs to the cytochrome c oxidase subunit 6A family.This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.

Protocols

Product Specific Protocols
WB protocol for COX6A1 antibody 11460-1-APDownload protocol
IHC protocol for COX6A1 antibody 11460-1-APDownload protocol
IF protocol for COX6A1 antibody 11460-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF

Autophagy

PLK1 (polo like kinase 1)-dependent autophagy facilitates gefitinib-induced hepatotoxicity by degrading COX6A1 (cytochrome c oxidase subunit 6A1).

Authors - Peihua Luo
  • KD Validated
humanWB

Elife

Function of hTim8a in complex IV assembly in neuronal cells provides insight into pathomechanism underlying Mohr-Tranebjærg syndrome.

Authors - Yilin Kang
humanWB

Cell Death Dis

O-GlcNAcylation on Rab3A attenuates its effects on mitochondrial oxidative phosphorylation and metastasis in hepatocellular carcinoma.

Authors - Weicheng Wu
humanWB

Mol Cell Proteomics

HIGD2A is Required for Assembly of the COX3 Module of Human Mitochondrial Complex IV

Authors - Daniella H Hock
mouseWB

Hum Mol Genet

Pathological characterization of a novel mouse model expressing the PD-linked CHCHD2-T61I mutation.

Authors - Teresa R Kee
humanWB

Mol Cell Proteomics

HIGD2A is Required for Assembly of the COX3 Module of Human Mitochondrial Complex IV.

Authors - Daniella H Hock