COX17 Polyclonal antibody

• Featured Product
• KD/KO Validated

COX17 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 11464-1-AP

Synonyms

COX 17

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Validation Data Gallery

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  WB analysis using 11464-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11464-1-AP (COX17 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 11464-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 11464-1-AP (COX17 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 11464-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11464-1-AP (COX17 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11464-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11464-1-AP (COX17 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11464-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using COX17 antibody (11464-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: MCF-7 cells, rat heart tissue, THP-1 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Product Information

11464-1-AP targets COX17 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COX17 fusion protein Ag2032
• Full Name: COX17 cytochrome c oxidase assembly homolog (S. cerevisiae)
• Calculated Molecular Weight: 63 aa, 7 kDa
• Observed Molecular Weight: 7 kDa
• GenBank Accession Number: BC010933
• Gene Symbol: COX17
• Gene ID (NCBI): 10063
• RRID: AB_2085109
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	Cell Stem Cell; Disrupting Mitochondrial Copper Distribution Inhibits Leukemic Stem Cell Self-Renewal.; Authors - Rashim Pal Singh ; KD Validated
human	IF	Am J Hum Genet; The mitochondrial disulfide relay system protein GFER is mutated in autosomal-recessive myopathy with cataract and combined respiratory-chain deficiency.; Authors - Di Fonzo Alessio A
human,rat	WB	Nutrients; Diosgenin Inhibits ROS Generation by Modulating NOX4 and Mitochondrial Respiratory Chain and Suppresses Apoptosis in Diabetic Nephropathy; Authors - Yujie Zhong
human,mouse	WB	FASEB J; Exercise improves angiogenic function of circulating exosomes in type 2 diabetes: Role of exosomal SOD3.; Authors - Kareem Abdelsaid
human	WB	J Biol Chem; An animal model for mitochondrial tyrosyl-tRNA synthetase deficiency reveals links between oxidative phosphorylation and retinal function.; Authors - Xiaofen Jin
mouse	WB	Toxicol Appl Pharmacol; Copper accumulation and compartmentalization in mouse fibroblast lacking metallothionein and copper chaperone, Atox1.; Authors - Miyayama Takamitsu T