COX10 Polyclonal antibody

• Featured Product
• KD/KO Validated

COX10 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 10611-2-AP

Synonyms

COX10, Heme O synthase

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Validation Data Gallery

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  WB analysis of A549 using 10611-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10611-2-AP (COX10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 10 publications below

Product Information

10611-2-AP targets COX10 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COX10 fusion protein Ag0930
• Full Name: COX10 homolog, cytochrome c oxidase assembly protein, heme A: farnesyltransferase (yeast)
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC006394
• Gene Symbol: COX10
• Gene ID (NCBI): 1352
• RRID: AB_2084833
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytochrome c oxidase (COX) is the terminal enzyme in the electron transfer chain located in the mitochondrial inner membrane. It catalyzes the electron transfer from reduced cytochrome c to oxygen. COX10 is predicted to contain 7-9 transmembrane domains, and it is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes heme A:farnesyltransferase, which is required for the expression of functional COX and functions in the maturation of the heme A prosthetic group of COX. In addition, COX10 may play a certain role in the development and progression of azoospermia.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; A deafness-associated tRNA mutation caused pleiotropic effects on the m1G37 modification, processing, stability and aminoacylation of tRNAIle and mitochondrial translation.; Authors - Feilong Meng
human	WB	Transl Res; Skeletal Muscle MiR-210 Expression is Associated with Mitochondrial Function in Peripheral Artery Disease Patients.; Authors - Ahmed Ismaeel
human	WB	Nat Commun; Mitochondrial respiration protects against oxygen-associated DNA damage.; Authors - Sung Ho Joong HJ
human	WB	EMBO Mol Med; Expression of the alternative oxidase complements cytochrome c oxidase deficiency in human cells.; Authors - Dassa Emmanuel P EP ; KD Validated
human	WB	Free Radic Biol Med; Mitochondrial and glucose metabolic dysfunctions in granulosa cells induce impaired oocytes of polycystic ovary syndrome through Sirtuin 3.; Authors - Qing Zhang
mouse	WB	Glia; Deficient mitochondrial respiration in astrocytes impairs trace fear conditioning and increases naloxone-precipitated aversion in morphine-dependent mice.; Authors - Kateryna Murlanova ; KO Validated