COX-1/Cyclooxygenase-1 Recombinant antibody

COX-1/Cyclooxygenase-1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241373C10

Cat no : 84151-4-RR

Synonyms

PGH1, PTGS1, PGHS-1, EC:1.14.99.1, Cyclooxygenase-1

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Validation Data Gallery

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  WB analysis using 84151-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84151-4-RR (COX-1/Cyclooxygenase-1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 84151-4-RR

  THP-1 cells were subjected to SDS PAGE followed by western blot with 84151-4-RR (COX-1/Cyclooxygenase-1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 84151-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84151-4-RR against Human COX-1/Cyclooxygenase-1 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: C2C12 cells, THP-1 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84151-4-RR is COX-1/Cyclooxygenase-1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: COX-1/Cyclooxygenase-1 fusion protein Ag4055
• Full Name: prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase)
• Calculated Molecular Weight: 599 aa, 69 kDa
• Observed Molecular Weight: 60-72 kDa
• GenBank Accession Number: BC029840
• Gene Symbol: PTGS1
• Gene ID (NCBI): 5742
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTGS1(Prostaglandin G/H synthase 1) belongs to the prostaglandin G/H synthase family and catalyzes the conversion of arachidonic acid to prostaglandin H2, which is subsequently metabolized to various biologically active prostaglandins. It is also named as COX1(Cyclooxygenase-1). There is no PTGS1 expression in fetal samples (prostate, seminal vesicles, or ejaculatory ducts), and only minimal expression in adult tissues (PMID: 10999846). PTGS1 has some isoforms with MW of 56-72 kD and PTGS1 can form homodimer.