COTL1 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

COTL1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10781-1-PBS

Synonyms

---

Validation Data Gallery

• 

  WB analysis using 10781-1-AP (same clone as 10781-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10781-1-AP (COTL1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10781-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 10781-1-AP (same clone as 10781-1-PBS)

  WB result of COTL1 antibody (10781-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COTL1 transfected HeLa cells. This data was developed using the same antibody clone with 10781-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse kidney using 10781-1-AP (same clone as 10781-1-PBS)

  IP result of anti-COTL1 (IP:10781-1-AP, 3ug; Detection:10781-1-AP 1:3000) with mouse kidney tissue lysate 12500ug. This data was developed using the same antibody clone with 10781-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 10781-1-AP (same clone as 10781-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10781-1-AP (COTL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10781-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 10781-1-AP (same clone as 10781-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10781-1-AP (COTL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10781-1-PBS in a different storage buffer formulation.

---

Product Information

10781-1-PBS targets COTL1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COTL1 fusion protein Ag1230
• Full Name: coactosin-like 1 (Dictyostelium)
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC010884
• Gene Symbol: COTL1
• Gene ID (NCBI): 23406
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

COTL1, also named as CLP, binds to F-actin in a calcium-independent manner. And it has no direct effect on actin depolymerization.