Validation Data Gallery
|Positive WB detected in||mouse heart tissue, human heart tissue, SGC-7901 cells, HeLa cells, mouse skeletal muscle tissue|
|Positive IP detected in||mouse skeletal muscle tissue|
|Positive IHC detected in||human cervical cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
14749-1-AP targets CORO1C in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, monkey|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CORO1C fusion protein Ag6488|
|Full Name||coronin, actin binding protein, 1C|
|Calculated molecular weight||53 kDa|
|Observed molecular weight||57 kDa|
|GenBank accession number||BC002342|
|Gene ID (NCBI)||23603|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
|Product Specific Protocols|
|WB protocol for CORO1C antibody 14749-1-AP||Download protocol|
|IHC protocol for CORO1C antibody 14749-1-AP||Download protocol|
|IF protocol for CORO1C antibody 14749-1-AP||Download protocol|
|IP protocol for CORO1C antibody 14749-1-AP||Download protocol|
|Click here to view our Standard Protocols|
A Novel Class of ER Membrane Proteins Regulates ER-Associated Endosome Fission.
J Cell Biol
Coronin 1C restricts endosomal branched actin to organize ER contact and endosome fission.
Hum Mol Genet
Proteomic analysis reveals co-ordinated alterations in protein synthesis and degradation pathways in LRRK2 knockout mice.
Front Mol Biosci
CORO1C is Associated With Poor Prognosis and Promotes Metastasis Through PI3K/AKT Pathway in Colorectal Cancer.
Coronin 1C, Regulated by Multiple microRNAs, Facilitates Cancer Cell Aggressiveness in Pancreatic Ductal Adenocarcinoma
microRNA‑196a‑3p inhibits cell proliferation and promotes cell apoptosis by targeting ADP ribosylation factor 4 in diffuse large B‑cell lymphoma.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Wen (Verified Customer) (12-22-2019)
Work well in western blot