CORIN Recombinant antibody

CORIN Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240339A10

Cat no : 83566-1-RR

Synonyms

TMPRSS10, serine peptidase, CRN, 240339A10

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Validation Data Gallery

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  WB analysis of rat heart using 83566-1-RR

  rat heart tissue were subjected to SDS PAGE followed by western blot with 83566-1-RR (CORIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 83566-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83566-1-RR (CORIN antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 83566-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83566-1-RR (CORIN antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83566-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83566-1-RR against Human CORIN were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: rat heart tissue
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83566-1-RR is CORIN Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CORIN fusion protein Ag15898
• Full Name: corin, serine peptidase
• Calculated Molecular Weight: 1042 aa, 117 kDa
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: BC110451
• Gene Symbol: CORIN
• Gene ID (NCBI): 10699
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CORIN is a transmembrane type II serine protease expressed in cardiomyocytes that converts pro-atrial natriuretic peptide into atrial natriuretic peptide, a cardiac hormone that regulates blood pressure. CORIN can be shed into the circulation through autocleavage and metalloproteinase-mediated hydrolysis processes of the membrane form of the protein. Three different soluble fragments derived from corin molecules, with molecular masses of ~180, ~160 and ~100 kDa were described in literature (PMID: 21288900, 29387201).