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  • KD/KO Validated

COQ7 Polyclonal antibody

COQ7 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 15083-1-AP

Synonyms

CAT5, CLK 1, CLK1, COQ7, Timing protein clk 1 homolog



Tested Applications

Positive WB detected inmouse liver tissue, mouse heart tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15083-1-AP targets COQ7 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COQ7 fusion protein Ag7161
Full Name coenzyme Q7 homolog, ubiquinone (yeast)
Calculated Molecular Weight 24 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC003185
Gene Symbol COQ7
Gene ID (NCBI) 10229
RRIDAB_2082207
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COQ7(Ubiquinone biosynthesis protein COQ7 homolog) catalyzes the production of coenzyme Q (CoQ) in mitochondria. The predicted protein contains 179 amino acids, is mostly helical, and contains an alpha-helical membrane insertion. It has a potential N-glycosylation site, a phosphorylation site for protein kinase C and another for casein kinase II, and 3 N-myristoylation sites. This protein has 2 isoforms produced by alternative splicing with MW 20-24 kDa.

Protocols

Product Specific Protocols
WB protocol for COQ7 antibody 15083-1-APDownload protocol
IHC protocol for COQ7 antibody 15083-1-APDownload protocol
IP protocol for COQ7 antibody 15083-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IF

Nat Cell Biol

A nuclear role for the respiratory enzyme CLK-1 in regulating mitochondrial stress responses and longevity.

Authors - Richard M Monaghan
humanWB

J Clin Invest

ADCK4 mutations promote steroid-resistant nephrotic syndrome through CoQ10 biosynthesis disruption.

Authors - Ashraf Shazia S
  • KD Validated
mouseWB

Nat Commun

Hepatic HuR modulates lipid homeostasis in response to high-fat diet.

Authors - Zhuojun Zhang
mouseWB

EMBO Mol Med

β-RA reduces DMQ/CoQ ratio and rescues the encephalopathic phenotype in Coq9 R239X mice.

Authors - Agustín Hidalgo-Gutiérrez
mouseWB

Redox Biol

The Q-junction and the inflammatory response are critical pathological and therapeutic factors in CoQ deficiency.

Authors - Pilar González-García
mouseWB

Acta Pharmacol Sin

Dysregulation of iron homeostasis and methamphetamine reward behaviors in Clk1-deficient mice.

Authors - Peng-Ju Yan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Ying (Verified Customer) (04-26-2021)

It's super clean and works great.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse and human fibroblasts, mouse tissues and human muscle biopsy samples