CSN2 Monoclonal antibody

CSN2 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF

Conjugate

Unconjugated

CloneNo.

2H10E6

Cat no : 66435-1-Ig

Synonyms

ALIEN, Alien homolog, COPS2, CSN2, SGN2, Signalosome subunit 2, TR interacting protein 15, TRIP 15, TRIP15

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 66435-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66435-1-Ig (CSN2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ROS1728 using 66435-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66435-1-Ig (CSN2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 66435-1-Ig

  C6 cells were subjected to SDS PAGE followed by western blot with 66435-1-Ig (CSN2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66435-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66435-1-Ig (CSN2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 66435-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66435-1-Ig (CSN2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 66435-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66435-1-Ig (COPS2 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human ovary tumor using 66435-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using CSN2 antibody (66435-1-Ig, Clone: 2H10E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, C6 cells, RAW 264.7 cells, ROS1728 cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human ovary tumor tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66435-1-Ig targets CSN2 in WB, IHC, IF applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CSN2 fusion protein Ag1415
• Full Name: COP9 constitutive photomorphogenic homolog subunit 2 (Arabidopsis)
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC012629
• Gene Symbol: COPS2
• Gene ID (NCBI): 9318
• RRID: AB_2881805
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COPS2 is an essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.