COPE Polyclonal antibody

COPE Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11457-1-AP

Synonyms

Epsilon-COP, Epsilon-coat protein, Coatomer subunit epsilon

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Validation Data Gallery

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  WB analysis of HEK-293 using 11457-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11457-1-AP (COPE antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 11457-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11457-1-AP (COPE antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11457-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11457-1-AP (COPE antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11457-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11457-1-AP (COPE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 11457-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11457-1-AP (COPE antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11457-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11457-1-AP (COPE antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 11457-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11457-1-AP (COPE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 11457-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11457-1-AP (COPE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 11457-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 11457-1-AP (COPE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11457-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11457-1-AP (COPE Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 11457-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11457-1-AP (COPE Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of rat stomach using 11457-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 11457-1-AP (COPE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11457-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11457-1-AP (COPE Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 11457-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11457-1-AP (COPE Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 11457-1-AP

  Immunofluorescent analysis of HepG2 cells, using COPE antibody 11457-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, PC-3 cells, A375 cells, HeLa cells, A431 cells, MCF-7 cells
• Positive IHC detected in: human stomach cancer tissue, human liver cancer tissue, human prostate cancer tissue, rat stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11457-1-AP targets COPE in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COPE fusion protein Ag2024
• Full Name: coatomer protein complex, subunit epsilon
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: BC017285
• Gene Symbol: COPE
• Gene ID (NCBI): 11316
• RRID: AB_11182386
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

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