COMP Polyclonal antibody

COMP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 28369-1-AP

Synonyms

TSP5, Thrombospondin-5, PSACH, Cartilage oligomeric matrix protein

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Validation Data Gallery

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  WB analysis using 28369-1-AP

  Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 28369-1-AP (COMP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 28369-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28369-1-AP (COMP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 28369-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28369-1-AP (COMP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 28369-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28369-1-AP (COMP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 28369-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using COMP antibody (28369-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, mouse cartilage tissue, rat cartilage tissue, pig cartilage tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

28369-1-AP targets COMP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COMP fusion protein Ag28749
• Full Name: cartilage oligomeric matrix protein
• Calculated Molecular Weight: 757 aa, 83 kDa
• Observed Molecular Weight: 80-110 kDa
• GenBank Accession Number: BC033676
• Gene Symbol: COMP
• Gene ID (NCBI): 1311
• RRID: AB_2881126
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COMP is a secreted pentameric glycoprotein built from modular units and belongs to the thrombospondin protein family. COMP is expressed by hypertrophic chondrocytes and osteoblasts during endochondral ossification, and its expression is increased during fracture healing. COMP is involved in the assembly of collagen fibrils and other structural matrix components. COMP interacts with a number of cartilage ECM proteins, including fibronectin, collagen I, II IX, XII, and XIV, matrilins, as well as proteoglycans such as aggrecan and others.

Publications

Species	Application	Title
human,mouse	WB	Int J Chron Obstruct Pulmon Dis; Identification of Hub Genes Associated with COPD Through Integrated Bioinformatics Analysis.; Authors - Lin Chen
human	WB	Reprod Sci; Effect of GLIS1 on the Lymph Node Metastasis of Cervical Squamous Carcinoma Based on Transcriptome Analysis; Authors - Jiazhen Huang
human	IHC	Front Mol Biosci; Serum proteomic profiling of precancerous gastric lesions and early gastric cancer reveals signatures associated with systemic inflammatory response and metaplastic differentiation; Authors - Yueqing Gong
mouse	IF	Eur J Pharmacol; Endonuclear Circ-calm4 Regulates Ferroptosis via a circR-Loop of the COMP Gene in Pulmonary Artery Smooth Muscle Cells; Authors - Aijing Liu