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COMMD5 Monoclonal antibody, PBS Only

COMMD5 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E10G6

Cat no : 67043-1-PBS

Synonyms

COMM domain containing 5, COMMD5, FLJ13008, HCARG, HT002

Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using COMMD5 Monoclonal antibody (67043-1-Ig)

    WB analysis of human heart using 67043-1-Ig (same clone as 67043-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 67043-1-Ig (COMMD5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67043-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat heart tissue using COMMD5 Monoclonal antibody (67043-1-Ig)

    WB analysis of rat heart using 67043-1-Ig (same clone as 67043-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 67043-1-Ig (COMMD5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67043-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using COMMD5 Monoclonal antibody (67043-1-Ig)

    WB analysis of MCF-7 using 67043-1-Ig (same clone as 67043-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67043-1-Ig (COMMD5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67043-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using COMMD5 Monoclonal antibody (67043-1-Ig)

    WB analysis of HEK-293 using 67043-1-Ig (same clone as 67043-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67043-1-Ig (COMMD5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67043-1-PBS in a different storage buffer formulation.

Product Information

67043-1-PBS targets COMMD5 in WB, Indirect ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen COMMD5 fusion protein Ag18464
Full Name COMM domain containing 5
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 15 kDa
GenBank Accession NumberBC003055
Gene Symbol COMMD5
Gene ID (NCBI) 28991
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

COMM domain containing 5 (COMMD5, synonyms: HCARG, HT002) is a hypertension-related calcium-regulated gene. COMMD5 is negatively regulated by extracellular calcium concentration, and its basal mRNA levels were higher in hypertensive animals. Tissue distribution of COMMD5 shows a preponderance in the heart, stomach, jejunum, kidney (tubular fraction), liver, and adrenal gland (mainly in the medulla). COMMD5 mRNA is significantly more expressed in adult than in fetal organs, and its levels are decreased in tumors and cancerous cell lines. COMMD5 protein has no transmembrane domain, but contains 67% -helix content, a calcium-binding site, four putative "leucine zipper" motifs, and a nuclear receptor-binding domain. At the subcellular level, COMMD5 shows a nuclear localization.