COL9A1 Polyclonal antibody

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  WB analysis using 12507-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12507-1-AP (Collagen Type IX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

COL9A1 Polyclonal Antibody for WB, ELISA

Cat No. 12507-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

COL9, Collagen alpha 1(IX) chain, Collagen alpha-1(IX) chain, Collagen Type IX, EDM6

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Tested Applications

• Positive WB detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12507-1-AP targets COL9A1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COL9A1 fusion protein Ag3189
• Full Name: collagen, type IX, alpha 1
• Calculated Molecular Weight: 921 aa 92 kDa
• Observed Molecular Weight: 64-67 kDa
• GenBank Accession Number: BC015409
• Gene Symbol: COL9A1
• Gene ID (NCBI): 1297
• RRID: AB_3669142
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P20849
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COL9A1 (collagen type IX alpha 1 chain), also known as MED. It is expected to be located in extracellular space, and the protein is enriched in the brain, choroid plexus and prostate. This gene encodes one of the three alpha chains of type IX collagen, which is a minor (5-20%) collagen component of hyaline cartilage. Type IX collagen is usually found in tissues containing type II collagen, a fibrillar collagen. Mutations in this gene are associated with osteoarthritis in humans, with multiple epiphyseal dysplasia, 6, a form of chondrodysplasia, and with Stickler syndrome, a disease characterized by ophthalmic, orofacial, articular, and auditory defects. The molecular weight of COL9A1 is 64 kDa, and it can recognize other isoforms.