Collagen Type VI Monoclonal antibody

Collagen Type VI Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

3H10B1

Cat no : 66824-1-Ig

Synonyms

COL6A1, Collagen alpha 1(VI) chain, Collagen Type VI, collagen, type VI, alpha 1, OPLL

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Validation Data Gallery

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  WB analysis of human skeletal muscle using 66824-1-Ig

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66824-1-Ig (Collagen Type VI antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 66824-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66824-1-Ig (Collagen Type VI antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66824-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66824-1-Ig (Collagen Type VI antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66824-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66824-1-Ig (Collagen Type VI antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66824-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66824-1-Ig (Collagen Type VI antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66824-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66824-1-Ig (Collagen Type VI antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human liver cancer using 66824-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Collagen Type VI antibody (66824-1-Ig, Clone: 3H10B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human liver cancer using 66824-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Collagen Type VI antibody (66824-1-Ig, Clone: 3H10B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human liver cancer using 66824-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Collagen Type VI antibody (66824-1-Ig, Clone: 3H10B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: human skeletal muscle tissue, human heart tissue
• Positive IHC detected in: human liver cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human liver cancer tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66824-1-Ig targets Collagen Type VI in WB, IHC, IF-P, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Collagen Type VI fusion protein Ag11809
• Full Name: collagen, type VI, alpha 1
• Calculated Molecular Weight: 1028 aa, 109 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC052575
• Gene Symbol: COL6A1
• Gene ID (NCBI): 1291
• RRID: AB_2882167
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Type VI Collagen is a major structural component of microfibrils. Type VI Collagen was found to be present throughout the connective tissue and in the extracellular matrix of cultured fibroblasts (PMID: 6723253). It is a heterotrimer composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI). The alpha-1(VI) chain is encoded by COL6A1 gene. Mutations in this gene result in Bethlem myopathy and Ullrich congenital muscular dystrophy.