COL6A1 Monoclonal antibody

COL6A1 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66824-1-Ig
Clone No.3H10B1

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

COL6, 3H10B1, collagen 6A1, Collagen alpha 1(VI) chain, Collagen alpha-1(VI) chain

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inhuman skeletal muscle tissue, human heart tissue
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66824-1-Ig targets COL6A1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen COL6A1 fusion protein Ag11809 Predict reactive species
Full Name collagen, type VI, alpha 1
Calculated Molecular Weight 1028 aa, 109 kDa
Observed Molecular Weight 140-150 kDa
GenBank Accession NumberBC052575
Gene Symbol COL6A1
Gene ID (NCBI) 1291
RRIDAB_2882167
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP12109
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Type VI Collagen is a major structural component of microfibrils. Type VI Collagen was found to be present throughout the connective tissue and in the extracellular matrix of cultured fibroblasts (PMID: 6723253). It is a heterotrimer composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI). The alpha-1(VI) chain is encoded by COL6A1 gene. Mutations in this gene result in Bethlem myopathy and Ullrich congenital muscular dystrophy.

Protocols

Product Specific Protocols
WB protocol for COL6A1 antibody 66824-1-IgDownload protocol
IHC protocol for COL6A1 antibody 66824-1-IgDownload protocol
IF protocol for COL6A1 antibody 66824-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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