Collagen Type IV Polyclonal antibody

Collagen Type IV Polyclonal Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 19674-1-AP

Synonyms

CA44, COL4A4, Collagen alpha 4(IV) chain, Collagen Type IV, collagen, type IV, alpha 4

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Validation Data Gallery

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  IF Staining of HeLa using 19674-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 19674-1-AP (Collagen Type IV antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

19674-1-AP targets Collagen Type IV in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, duck
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: collagen, type IV, alpha 4
• Calculated Molecular Weight: 164 kDa
• GenBank Accession Number: NM_000092
• Gene Symbol: COL4A4
• Gene ID (NCBI): 1286
• RRID: AB_2878595
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COL4A4 belongs to the type IV collagen family. COL4A4 is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Defects in COL4A4 are a cause of Alport syndrome autosomal recessive (APSAR). Defects in COL4A4 are a cause of benign familial hematuria (BFH). This antibody is specific to COL4A4.

Publications

Species	Application	Title
rat	WB	J Ethnopharmacol; In silico prediction and experimental validation to reveal the protective mechanism of Puerarin against excessive extracellular matrix accumulation through inhibiting ferroptosis in diabetic nephropathy; Authors - Biyu Hou
	WB	PLoS Negl Trop Dis; Mining host candidate regulators of schistosomiasis-induced liver fibrosis in response to artesunate therapy through transcriptomics approach; Authors - Yajie Yuan
rat	WB	Eur J Pharmacol; Sirt1 activation prevents anti-Thy 1.1 mesangial proliferative glomerulonephritis in the rat through the Nrf2/ARE pathway.; Authors - Kaipeng Huang
rat	IF	J Chem Neuroanat; Berberine protects against chronic cerebral hypoperfusion-induced cognitive impairment and hippocampal damage via regulation of the ERK/Nrf2 pathway.; Authors - Ningqun Wang
duck	WB	Poult Sci; Co-exposure of polyvinyl chloride microplastics with cadmium promotes nonalcoholic fatty liver disease in female ducks through oxidative stress and glycolipid accumulation; Authors - Yan Chen
human	IF	iScience; Discovery of non-genomic drivers of YAP signaling modulating the cell plasticity in CRC tumor lines; Authors - Nobuhiko Ogasawara