COL3A1 Monoclonal antibody

COL3A1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1


Human, Rabbit, Pig, rat, mouse and More (1)







Cat no : 68320-1-Ig


COL3A1, Collagen alpha 1(III) chain, collagen, type III, alpha 1, EDS4A, FLJ34534

Tested Applications

Positive WB detected inpig colon tissue, human placenta tissue, human rectum tissue, rabbit large intestine tissue, rat colon tissue
Positive IHC detected inhuman ovary tumor tissue, mouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68320-1-Ig targets COL3A1 in WB, IHC, ELISA applications and shows reactivity with Human, Rabbit, Pig, rat, mouse samples.

Tested Reactivity Human, Rabbit, Pig, rat, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen COL3A1 fusion protein Ag4229
Full Name collagen, type III, alpha 1
Calculated Molecular Weight 1466 aa, 139 kDa
Observed Molecular Weight130 kDa
GenBank Accession NumberBC028178
Gene Symbol COL3A1
Gene ID (NCBI) 1281
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Type III collagen is a fibrillar-forming collagen comprising three α1(III) chains and is expressed in early embryos and throughout embryogenesis (PMID: 9050868). In the adult, type III collagen is a major component of the extracellular matrix in a variety of internal organs and skin. It occurs in most soft connective tissues along with type I collagen (PMID: 2445760). COL3A1 gene encodes type III procollagen. Mutations in this gene are associated with Ehlers-Danlos syndrome type IV, and with aortic and arterial aneurysms (PMID: 10706896; 2243125; 18389341).


Product Specific Protocols
WB protocol for COL3A1 antibody 68320-1-IgDownload protocol
IHC protocol for COL3A1 antibody 68320-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Biochem Biophys Res Commun

The characterization of the sensitive ovarian cancer cell lines A2780 and W1 in response to ovarian CAFs

Authors - Karolina Wojtowicz

Int J Pharm

Metformin suppressed tendon injury-induced adhesion via hydrogel-nanoparticle sustained-release system

Authors - Zhi Jie Li

J Transl Med

TNFSF14/LIGHT promotes cardiac fibrosis and atrial fibrillation vulnerability via PI3Kγ/SGK1 pathway-dependent M2 macrophage polarisation

Authors - Yirong Wu

Cancer Biol Ther

Role and mechanism of COL3A1 in regulating the growth, metastasis, and drug sensitivity in cisplatin-resistant non-small cell lung cancer cells

Authors - Jiankun Ren
  • KD Validated