COL17A1 Polyclonal antibody, PBS Only

COL17A1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 31190-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 31190-1-AP (same clone as 31190-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31190-1-AP (COL17A1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31190-1-PBS in a different storage buffer formulation.

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  IHC staining of rat skin using 31190-1-AP (same clone as 31190-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 31190-1-AP (COL17A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31190-1-PBS in a different storage buffer formulation.

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  IHC staining of rat skin using 31190-1-AP (same clone as 31190-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 31190-1-AP (COL17A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31190-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 31190-1-AP (same clone as 31190-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using COL17A1 antibody (31190-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31190-1-PBS in a different storage buffer formulation.

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  IF Staining of HaCaT using 31190-1-AP (same clone as 31190-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using COL17A1 antibody (31190-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31190-1-PBS in a different storage buffer formulation.

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Product Information

31190-1-PBS targets COL17A1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COL17A1 fusion protein Ag34645
• Full Name: collagen, type XVII, alpha 1
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: BC168368
• Gene Symbol: COL17A1
• Gene ID (NCBI): 1308
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Collagen alpha-1(XVII) chain, also termed 180-kDa bullous pemphigoid antigen (BP180), is a type II transmembrane glycoprotein with a collagenous carboxyl-terminal extracellular domain and a noncollagenous amino-terminal cytoplasmic domain. High expression of COL17A1 has also been identified as a marker of human esophageal quiescent stem/progenitor cells (PMID: 35709398, 9545306). As a structural component of the hemidesmosome, COL17A1 is not only present as the full-length 180 kDa transmembrane form, but also as a cleaved 120 kDa soluble form that is further processed to a 97 kDa form (PMID: 113594550).