COG7 Polyclonal antibody

COG7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Reviews (1)

Cat no : 13552-1-AP

Synonyms

CDG2E, COG complex subunit 7, COG7

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Validation Data Gallery

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  WB analysis using 13552-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13552-1-AP (COG7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 13552-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 13552-1-AP (COG7 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 13552-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13552-1-AP (COG7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 13552-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13552-1-AP (COG7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, Y79 cells, HeLa cells
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13552-1-AP targets COG7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COG7 fusion protein Ag4335
• Full Name: component of oligomeric golgi complex 7
• Calculated Molecular Weight: 770 aa, 86 kDa
• Observed Molecular Weight: 86 kDa
• GenBank Accession Number: BC037563
• Gene Symbol: COG7
• Gene ID (NCBI): 91949
• RRID: AB_2229608
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (09-28-2020)

This antibody recognised a clean band at the expected molecular weight.

• Applications: Western Blot