COG6 Polyclonal antibody, PBS Only

COG6 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14277-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 14277-1-AP (same clone as 14277-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14277-1-AP (COG6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14277-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 14277-1-AP (same clone as 14277-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 14277-1-AP (COG6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14277-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 14277-1-AP (same clone as 14277-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14277-1-AP (COG6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14277-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 14277-1-AP (same clone as 14277-1-PBS)

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 14277-1-AP (COG6 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14277-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 14277-1-AP (same clone as 14277-1-PBS)

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 14277-1-AP (COG6 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14277-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 14277-1-AP (same clone as 14277-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using COG6 antibody 14277-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14277-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 14277-1-AP (same clone as 14277-1-PBS)

  Immunofluorescent analysis of Hela cells, using COG6 antibody 14277-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 14277-1-PBS in a different storage buffer formulation.

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Product Information

14277-1-PBS targets COG6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COG6 fusion protein Ag5624
• Full Name: component of oligomeric golgi complex 6
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 73 kDa
• GenBank Accession Number: BC051723
• Gene Symbol: COG6
• Gene ID (NCBI): 57511
• RRID: AB_10694426
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.