COBLL1 Polyclonal antibody

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  WB analysis using 31191-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31191-1-AP (COBLL1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 31191-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31191-1-AP (COBLL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 31191-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31191-1-AP (COBLL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 31191-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31191-1-AP (COBLL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HaCaT using 31191-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HaCaT cells using COBLL1 antibody (31191-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

COBLL1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 31191-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

KIAA0977, Cordon bleu protein like 1, COBLR1, COBL like 1

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Tested Applications

• Positive WB detected in: A431 cells
• Positive IHC detected in: mouse kidney tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HaCaT cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31191-1-AP targets COBLL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COBLL1 fusion protein Ag34853
• Full Name: COBL-like 1
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: BC071588
• Gene Symbol: COBLL1
• Gene ID (NCBI): 22837
• RRID: AB_3669891
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q53SF7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cordon-blue protein-like 1 (COBLL1), a negative regulator of apoptosis, is associated with lower insulin resistance. COBLL1 is involved in the cancer cell morphogenesis to a neuron-like cell shape observed in the CRPC model cells, promoting cell growth and migration (PMID: 29686105, 29686105). COBLL1 is highly expressed in most human tissues including lung, liver, kidney, pancreas, ovary, spinal cord and brain.