CNTROB Monoclonal antibody, PBS Only

CNTROB Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D12A4

Cat no : 67061-1-PBS

Synonyms

Centrobin, CNTROB, LIP8, LYST interacting protein 8, PP1221

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Validation Data Gallery

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  WB analysis using 67061-1-Ig (same clone as 67061-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67061-1-Ig (CNTROB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67061-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 67061-1-Ig (same clone as 67061-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67061-1-Ig (CNTROB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67061-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 67061-1-Ig (same clone as 67061-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67061-1-Ig (CNTROB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67061-1-PBS in a different storage buffer formulation.

• 

  IF Staining of hTERT-RPE1 using 67061-1-Ig (same clone as 67061-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CNTROB antibody (67061-1-Ig, Clone: 3D12A4 ) at dilution of 1:350 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), ARL13B antibody (17711-1-AP, red). This data was developed using the same antibody clone with 67061-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67061-1-PBS targets CNTROB in WB, IF, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CNTROB fusion protein Ag25447
• Full Name: centrobin, centrosomal BRCA2 interacting protein
• Calculated Molecular Weight: 903 aa, 101 kDa
• Observed Molecular Weight: 97 kDa
• GenBank Accession Number: BC021134
• Gene Symbol: CNTROB
• Gene ID (NCBI): 116840
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.