CNRIP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CNRIP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 16827-1-AP

Synonyms

C2orf32, CNRIP1, CRIP 1, CRIP1

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Validation Data Gallery

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  WB analysis of mouse brain using 16827-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16827-1-AP (CNRIP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16827-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16827-1-AP (CNRIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 16827-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 16827-1-AP (CNRIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16827-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16827-1-AP (CNRIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16827-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16827-1-AP (CNRIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 16827-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16827-1-AP (CNRIP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 16827-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16827-1-AP (CNRIP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue, human brain tissue
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below

Product Information

16827-1-AP targets CNRIP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CNRIP1 fusion protein Ag10428
• Full Name: cannabinoid receptor interacting protein 1
• Calculated Molecular Weight: 164 aa, 19 kDa
• Observed Molecular Weight: 13-25 kDa
• GenBank Accession Number: BC011535
• Gene Symbol: CNRIP1
• Gene ID (NCBI): 25927
• RRID: AB_2245126
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CNRIP1, also named as C2orf32, has two isoforms with MW 13-25 kDa.

Publications

Species	Application	Title
human,mouse	WB,IHC	Hepatology; DNA Methylation of Cannabinoid Receptor Interacting Protein 1 Promotes Pathogenesis of Intrahepatic Cholangiocarcinoma Through Suppressing Parkin-Dependent Pyruvate Kinase M2 Ubiquitination.; Authors - Diyu Chen
mouse	WB,IHC	Brain Res Bull; Disrupting cannabinoid receptor interacting protein 1 rescues cognitive flexibility in long-term estrogen-deprived female mice.; Authors - Fu Yang ; KO Validated