Cannabinoid receptor 1 Polyclonal antibody

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  WB analysis using 29708-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29708-1-AP (Cannabinoid receptor 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29708-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29708-1-AP (Cannabinoid receptor 1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29708-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 29708-1-AP (Cannabinoid receptor 1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

Cannabinoid receptor 1 Polyclonal Antibody for WB, ELISA

Cat No. 29708-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, ELISA

Synonyms

CANN6, Cannabinoid receptor 1, cannabinoid receptor 1 (brain), CB R, CB1, CB1A, CB1K5, CB1R, CNR, CNR1

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Tested Applications

• Positive WB detected in: mouse brain tissue, SH-SY5Y cells, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

29708-1-AP targets Cannabinoid receptor 1 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cannabinoid receptor 1 fusion protein Ag31838
• Full Name: cannabinoid receptor 1 (brain)
• Calculated Molecular Weight: 472 aa, 53 kDa
• Observed Molecular Weight: 48 kDa, 66 kDa
• GenBank Accession Number: BC074812
• Gene Symbol: Cannabinoid receptor 1
• Gene ID (NCBI): 1268
• RRID: AB_2918340
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P21554
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cannabinoid receptor 1 (CNR1, or CB1) and CNR2 (CB2) are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family. The CB1 receptor is expressed mainly in the brain. The CB2 receptor is expressed mainly in the immune system and in hematopoietic cells. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana.

Publications

Species	Application	Title
mouse	WB	ACS Chem Neurosci; Basolateral Amygdala Cannabinoid CB1 Receptor Controls Formation and Elimination of Social Fear Memory; Authors - Ming Li ; KO Validated