CNPase Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

CNPase Monoclonal Antibody for WB, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1B9G9

Cat no : 66729-1-PBS

Synonyms

CNP, EC:3.1.4.37, 2',3'-cyclic-nucleotide 3'-phosphodiesterase, 2',3' cyclic nucleotide 3' phosphodiesterase, 1B9G9

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Validation Data Gallery

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  WB analysis using 66729-1-Ig (same clone as 66729-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66729-1-Ig (CNPase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66729-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66729-1-Ig (same clone as 66729-1-PBS)

  WB result of CNPase antibody (66729-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNPase transfected HepG2 cells. This data was developed using the same antibody clone with 66729-1-PBS in a different storage buffer formulation.

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  WB analysis of U-251 using 66729-1-Ig (same clone as 66729-1-PBS)

  U-251 cells were subjected to SDS PAGE followed by western blot with 66729-1-Ig (CNPase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66729-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 66729-1-Ig (same clone as 66729-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66729-1-Ig (CNPase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66729-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 66729-1-Ig (same clone as 66729-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 66729-1-Ig (CNPase antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66729-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 66729-1-Ig (same clone as 66729-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human CNPase (66729-1-Ig, Clone:1B9G9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66729-1-PBS in a different storage buffer formulation.

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Product Information

66729-1-PBS targets CNPase in WB, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CNPase fusion protein Ag4227
• Full Name: 2',3'-cyclic nucleotide 3' phosphodiesterase
• Calculated Molecular Weight: 421 aa, 48 kDa
• Observed Molecular Weight: 45-48 kDa
• GenBank Accession Number: BC028040
• Gene Symbol: CNPase
• Gene ID (NCBI): 1267
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CNP(2',3'-cyclic-nucleotide 3'-phosphodiesterase) belongs to the cyclic nucleotide phosphodiesterase family. It plays an essential role in axonal survival but not in myelin assembly, coupling oligodendroglial functions in axonal support and myelination. This protein has 2 isoforms produced by alternative splicing with molecular mass of 45 and 48 kDa.