CNPase Polyclonal antibody

CNPase Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 13427-1-AP

Synonyms

CNP, EC:3.1.4.37, 2',3'-cyclic-nucleotide 3'-phosphodiesterase, 2',3' cyclic nucleotide 3' phosphodiesterase

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Validation Data Gallery

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  WB analysis using 13427-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13427-1-AP (CNPase antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13427-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13427-1-AP (CNPase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 13427-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13427-1-AP (CNPase antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse brain using 13427-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13427-1-AP (CNPase antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse brain using 13427-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 13427-1-AP (CNPase antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 13427-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 13427-1-AP (CNPase antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 13427-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human CNPase (13427-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 8 publications below
• IF: See 4 publications below

Product Information

13427-1-AP targets CNPase in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CNPase fusion protein Ag4227
• Full Name: 2',3'-cyclic nucleotide 3' phosphodiesterase
• Calculated Molecular Weight: 421 aa, 48 kDa
• Observed Molecular Weight: 45-48 kDa
• GenBank Accession Number: BC028040
• Gene Symbol: CNPase
• Gene ID (NCBI): 1267
• RRID: AB_2082477
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CNP(2',3'-cyclic-nucleotide 3'-phosphodiesterase) belongs to the cyclic nucleotide phosphodiesterase family. It plays an essential role in axonal survival but not in myelin assembly, coupling oligodendroglial functions in axonal support and myelination. This protein has 2 isoforms produced by alternative splicing with molecular mass of 45 and 48 kDa.

Publications

Species	Application	Title
mouse	IF	Nat Neurosci; TREM2 interacts with TDP-43 and mediates microglial neuroprotection against TDP-43-related neurodegeneration.; Authors - Manling Xie
mouse	IF	J Clin Invest; Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.; Authors - Zhixiong Liu
mouse	WB,IHC	J Pineal Res; Circadian misalignment impairs oligodendrocyte myelination via Bmal1 overexpression leading to anxiety and depression-like behaviors; Authors - Yao Zuo
mouse	WB	Acta Physiol (Oxf); Keratin 1 attenuates hypoxic pulmonary artery hypertension by suppressing pulmonary artery media smooth muscle expansion.; Authors - Li Zhang
mouse	IHC	Mol Nutr Food Res; A High MCT-Based Ketogenic Diet Suppresses Th1 and Th17 Responses to Ameliorate Experimental Autoimmune Encephalomyelitis in Mice by Inhibiting GSDMD and JAK2-STAT3/4 Pathways; Authors - Qianye Zhang
rat	IHC,IF	Front Cell Neurosci; Neural Stem Cells Overexpressing Nerve Growth Factor Improve Functional Recovery in Rats Following Spinal Cord Injury via Modulating Microenvironment and Enhancing Endogenous Neurogenesis.; Authors - Lei Wang