CNOT8 Recombinant antibody

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  WB analysis using 86091-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86091-1-RR (CNOT8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86091-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86091-1-RR against Human CNOT8 were performed. The affinity constant is 0.726 nM.

CNOT8 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 86091-1-RR

Clone No.250482E2

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

CAF1, CAF1 like protein, Caf1b, CAF1-like protein, CAF2

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, HL-60 cells, U-251 cells, rat testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86091-1-RR targets CNOT8 in WB, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CNOT8 fusion protein Ag1201
• Full Name: CCR4-NOT transcription complex, subunit 8
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC017366
• Gene Symbol: CNOT8
• Gene ID (NCBI): 9337
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UFF9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCR4-NOT transcription complex subunit 8(CNOT8), is a ubiquitous transcription factor, which required for a diverse set of processes. The CCR4-NOT complex is a global regulator of RNA polymerase II, and functions as general transcription regulation complex. It consisted part by CCR4, NOT1 to NOT5, and CAF1.