CNOT8 Polyclonal antibody

• Featured Product
• KD/KO Validated

CNOT8 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 10752-1-AP

Synonyms

CAF1, CAF1 like protein, CAF2, CALIF, CALIFp, CCR4 associated factor 8, CNOT8, hCAF1, POP2

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Validation Data Gallery

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  WB analysis of Jurkat using 10752-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10752-1-AP (CNOT8 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10752-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10752-1-AP (CNOT8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 10752-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10752-1-AP (CNOT8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 10752-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10752-1-AP (CNOT8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 10752-1-AP

  IP Result of anti-CNOT8 (IP:10752-1-AP, 4ug; Detection:10752-1-AP 1:600) with mouse testis tissue lysate 4000ug.

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  IHC staining of human prostate cancer using 10752-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10752-1-AP (CNOT8 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human prostate cancer using 10752-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10752-1-AP (CNOT8 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 10752-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10752-1-AP (CNOT8 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 10752-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10752-1-AP (CNOT8 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 10752-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10752-1-AP (CNOT8 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 10752-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 10752-1-AP (CNOT8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, mouse testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human prostate cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Product Information

10752-1-AP targets CNOT8 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CNOT8 fusion protein Ag1201
• Full Name: CCR4-NOT transcription complex, subunit 8
• Calculated molecular weight: 34 kDa
• Observed molecular weight: 34 kDa
• GenBank accession number: BC017366
• Gene symbol: CNOT8
• Gene ID (NCBI): 9337
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCR4-NOT transcription complex subunit 8(CNOT8), is a ubiquitous transcription factor, which required for a diverse set of processes. The CCR4-NOT complex is a global regulator of RNA polymerase II, and functions as general transcription regulation complex. It consisted part by CCR4, NOT1 to NOT5, and CAF1. This is a rabbit polyclonal antibody raised against the full-length chain of human CNOT8.

Publications

Species	Application	Title
human	WB	Mol Cell; Mechanism of ribosome-associated mRNA degradation during tubulin autoregulation; Authors - Markus Höpfler ; KD Validated
human	WB	Mol Biosyst; Large-scale investigation of oxygen response mutants in Saccharomyces cerevisiae.; Authors - Samanfar Bahram B ; KD Validated
mouse	WB	Cells; Translation Efficiency and Degradation of ER-Associated mRNAs Modulated by ER-Anchored poly(A)-Specific Ribonuclease (PARN).; Authors - Tian-Li Duan
human	WB	Nucleic Acids Res; BESST: a novel LncRNA knockout strategy with less genome perturbance; Authors - Shikuan Zhang
human	WB,IF	Cells; Disruption of the Mammalian Ccr4-Not Complex Contributes to Transcription-Mediated Genome Instability; Authors - Nafiseh Chalabi Hagkarim ; KD Validated