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  • KD/KO Validated

CNOT8 Polyclonal antibody

CNOT8 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 10752-1-AP


CAF1, CAF1 like protein, CAF2, CALIF, CALIFp, CCR4 associated factor 8, CNOT8, hCAF1, POP2

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, mouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman prostate cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10752-1-AP targets CNOT8 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNOT8 fusion protein Ag1201
Full Name CCR4-NOT transcription complex, subunit 8
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC017366
Gene Symbol CNOT8
Gene ID (NCBI) 9337
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCR4-NOT transcription complex subunit 8(CNOT8), is a ubiquitous transcription factor, which required for a diverse set of processes. The CCR4-NOT complex is a global regulator of RNA polymerase II, and functions as general transcription regulation complex. It consisted part by CCR4, NOT1 to NOT5, and CAF1. This is a rabbit polyclonal antibody raised against the full-length chain of human CNOT8.


Product Specific Protocols
WB protocol for CNOT8 antibody 10752-1-APDownload protocol
IHC protocol for CNOT8 antibody 10752-1-APDownload protocol
IF protocol for CNOT8 antibody 10752-1-APDownload protocol
IP protocol for CNOT8 antibody 10752-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Mol Cell

Mechanism of ribosome-associated mRNA degradation during tubulin autoregulation

Authors - Markus Höpfler
  • KD Validated

Mol Biosyst

Large-scale investigation of oxygen response mutants in Saccharomyces cerevisiae.

Authors - Samanfar Bahram B
  • KD Validated


Translation Efficiency and Degradation of ER-Associated mRNAs Modulated by ER-Anchored poly(A)-Specific Ribonuclease (PARN).

Authors - Tian-Li Duan

Nucleic Acids Res

BESST: a novel LncRNA knockout strategy with less genome perturbance

Authors - Shikuan Zhang


Disruption of the Mammalian Ccr4-Not Complex Contributes to Transcription-Mediated Genome Instability

Authors - Nafiseh Chalabi Hagkarim
  • KD Validated


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Roy (Verified Customer) (06-12-2024)

Works well to detect CNOT8 by Western Blot (1/500 Dilution)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/500
  • Cell Tissue Type: HeLa