CNOT4 Recombinant antibody, PBS Only (Capture)

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  WB analysis using 83615-4-RR (same clone as 83615-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83615-4-RR (CNOT4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83615-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83615-4-RR (same clone as 83615-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83615-4-RR (CNOT4 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83615-4-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83615-4-RR (same clone as 83615-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CNOT4 antibody (83615-4-RR, Clone: 240559E1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83615-4-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83615-4-RR (same clone as 83615-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cnot4 Recombinant Antibody (83615-4-RR, Clone:240559E1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83615-4-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00593-1

  Cytometric bead array standard curve of MP00593-1, CNOT4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83615-4-PBS. Detection antibody: 83615-3-PBS. Standard: Ag3163. Range: 0.313-40 ng/mL.

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  Cytometric bead array standard curve of MP00593-2

  Cytometric bead array standard curve of MP00593-2, CNOT4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83615-4-PBS. Detection antibody: 83615-1-PBS. Standard: Ag3163. Range: 1.25-40 ng/mL.

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  Affinity and Kinetic Characterization of 83615-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83615-4-RR against Human CNOT4 were performed. The affinity constant is 0.485 nM.

CNOT4 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 83615-4-PBS

Clone No.240559E1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

E3 ubiquitin-protein ligase CNOT4, CLONE243, CCR4-NOT transcription complex subunit 4, CCR4-associated factor 4, CCR4 associated factor 4

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Product Information

83615-4-PBS targets CNOT4 as part of a matched antibody pair:

MP00593-1: 83615-4-PBS capture and 83615-3-PBS detection (validated in Cytometric bead array)

MP00593-2: 83615-4-PBS capture and 83615-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CNOT4 fusion protein Ag3163
• Full Name: CCR4-NOT transcription complex, subunit 4
• Calculated Molecular Weight: 575 aa, 64 kDa
• Observed Molecular Weight: 64-85 kDa
• GenBank Accession Number: BC035590
• Gene Symbol: CNOT4
• Gene ID (NCBI): 4850
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O95628
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CNOT4, also known as CCR4-NOT transcription complex subunit 4 , has E3 ubiquitin ligase activity, and promotes ubiquitination and degradation of target proteins. CNOT4 is involved in activation of the JAK/STAT pathway. CNOT4 exist many isoforms and the range of its molecular weight from 48 to 84 kDa.