CNOT3 Monoclonal antibody, PBS Only

CNOT3 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig , mouse, rat , rabbit

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E12C6

Cat no : 68436-1-PBS

Synonyms

CCR4 associated factor 3, CNOT3, KIAA0691, LENG2, NOT3, NOT3H

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Validation Data Gallery

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  WB analysis using 68436-1-Ig (same clone as 68436-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68436-1-Ig (CNOT3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68436-1-PBS in a different storage buffer formulation.

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Product Information

68436-1-PBS targets CNOT3 in WB, Indirect ELISA applications and shows reactivity with Human, pig , mouse, rat , rabbit samples.

• Tested Reactivity: Human, pig , mouse, rat , rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CNOT3 fusion protein Ag33646
• Full Name: CCR4-NOT transcription complex, subunit 3
• Calculated Molecular Weight: 120 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC016474
• Gene Symbol: CNOT3
• Gene ID (NCBI): 4849
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The CCR4-NOT complex, the major deadenylase in mammalian cells, shortens the mRNA poly(A) tail and leads to the destabilization of mRNAs. The CCR4-NOT complex plays pivotal roles in various physiological functions, including cell apoptosis, proliferation, and metabolism [PMID:22342980]. CNOT3, belongs to the CCR4-NOT complex, has a critical role in maintaining mouse and human ESC identity as a protein complex and inhibit differentiation into the extraembryonic lineages. It is also involved in the regulation of the spindle assembly checkpoint [PMID:22342980,22367759].