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CNOT2 Polyclonal antibody
CNOT2 Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 10313-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | NIH/3T3 cells, HEK-293 cells |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 8 publications below |
| IF | See 1 publications below |
Product Information
10313-1-AP targets CNOT2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CNOT2 fusion protein Ag0347 |
| Full Name | CCR4-NOT transcription complex, subunit 2 |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC002597 |
| Gene Symbol | CNOT2 |
| Gene ID (NCBI) | 4848 |
| RRID | AB_2245072 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The Ccr4-Not complex is evolutionarily conserved and controls gene expression both at the level of transcription initiation and of mRNA degradation. And this complex can regulate pol II transcription. Promoter targeting of CCR4-NOT transcription complex subunit 2 (CNOT2) results in strong repression of pol II transcription. CNOT2-mediated repression is sensitive to the HDAC (histone deacetylase) inhibitor, TSA (trichostatin A).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CNOT2 antibody 10313-1-AP | Download protocol |
| IF protocol for CNOT2 antibody 10313-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Stem Cells Cnot1, Cnot2, and Cnot3 maintain mouse and human ESC identity and inhibit extraembryonic differentiation.
| ||
RNA Biol Essential functions of the CNOT7/8 catalytic subunits of the CCR4-NOT complex in mRNA regulation and cell viability. | ||
Stem Cells Dev Identification of Ccr4-Not complex components as regulators of transition from partial to genuine induced pluripotent stem cells.
| ||
bioRxiv Cnot3 is required for male germ cell development and spermatogonial stem cell maintenance | ||
Methods Mol Biol Dissecting the Role of the Ccr4-Not Deadenylase Complex in Pluripotency and Differentiation |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Aditya (Verified Customer) (10-08-2024) | very clean blot
|
FH Abe (Verified Customer) (04-25-2024) | Worked well for IF at 1:100 overnight at 4c in RPE-1 cells.
|
FH Ayse (Verified Customer) (06-08-2022) | Used for IF experiment (dilution 1/100) and WB experiment (1/2000). The antibody works well for the 2 kind of experiment. In few weeks, I will also test for IP.
 |
