CNNM4 Polyclonal antibody

CNNM4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14066-1-AP

Synonyms

KIAA1592, Cyclin-M4, cyclin M4, Ancient conserved domain-containing protein 4, ACDP4

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Validation Data Gallery

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  WB analysis using 14066-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14066-1-AP (CNNM4 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 14066-1-AP

  IP result of anti-CNNM4 (IP:14066-1-AP, 4ug; Detection:14066-1-AP 1:1000) with mouse testis tissue lysate 4400ug.

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  IHC staining of human colon using 14066-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14066-1-AP (CNNM4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 14066-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CNNM4 antibody (14066-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse colon tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

14066-1-AP targets CNNM4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CNNM4 fusion protein Ag5121
• Full Name: cyclin M4
• Calculated Molecular Weight: 87 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC063295
• Gene Symbol: CNNM4
• Gene ID (NCBI): 26504
• RRID: AB_2878009
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ancient conserved domain-containing protein 4/cyclin M4 (CNNM4), a membrane protein that stimulates Mg2+ efflux, binds phosphatase of regenerating liver (PRL), which is frequently overexpressed in malignant human cancers (PMID: 25347473). CNNM4 is predominant in the brain, bone marrow, immune system, and especially abundant in the intestinal tract. CNNM4 localizes at the basolateral membrane of the intestinal epithelia and mediates transcellular Mg2+ transport (PMID: 27006114).

Publications

Species	Application	Title
mouse	WB,IHC	Nat Commun; Restoring cellular magnesium balance through Cyclin M4 protects against acetaminophen-induced liver damage; Authors - Irene González-Recio