CNN3 Polyclonal antibody

• Featured Product
• KD/KO Validated

CNN3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 11509-1-AP

Synonyms

CNN 3, Calponin-3, Calponin3, Calponin 3

---

Validation Data Gallery

• 

  WB analysis of HEK-293 using 11509-1-AP

  WB result of CNN3 antibody (11509-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNN3 transfected HEK-293 cells.

• 

  WB analysis of human kidney using 11509-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11509-1-AP (CNN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HAP1 cells using 11509-1-AP

  HAP1 (WT and CNN3 KO) lysates prepared with RIPA buffer, 20 μg of protein loaded. 11509-1-AP incubated at 1:100 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown for each blot. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.

• 

  WB analysis using 11509-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11509-1-AP (CNN3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 11509-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11509-1-AP (CNN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of U-87MG cells using 11509-1-AP

  U-87MG lysates prepared and IP of APP performed using 2.0 μg of 11509-1-AP coupled to protein A- Dynabeads beads. The Ponceau stained transfers of each blot are shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitated; HC = antibody heavy chain. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.

• 

  IP experiment of HEK-293 using 11509-1-AP

  IP result of anti-CNN3 (IP:11509-1-AP, 4ug; Detection:11509-1-AP 1:15000) with HEK-293 cells lysate 960 ug.

• 

  IHC staining of human prostate cancer using 11509-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11509-1-AP (CNN3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11509-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11509-1-AP (CNN3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 11509-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11509-1-AP (CNN3 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin using 11509-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 11509-1-AP (CNN3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 11509-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 11509-1-AP (CNN3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human hysteromyoma using 11509-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 11509-1-AP (CNN3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hysteromyoma using 11509-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 11509-1-AP (CNN3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 11509-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CNN3 antibody (11509-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

---

Tested Applications

• Positive WB detected in: HCT 116 cells, HAP1 cells, human kidney tissue, human skeletal muscle tissue, HEK-293 cells, HepG2 cells, NIH/3T3 cells
• Positive IP detected in: HEK-293 cells, U-87MG cells
• Positive IHC detected in: human prostate cancer tissue, human hysteromyoma tissue, human skin tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

11509-1-AP targets CNN3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CNN3 fusion protein Ag2068
• Full Name: calponin 3, acidic
• Calculated Molecular Weight: 329 aa, 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC025372
• Gene Symbol: CNN3
• Gene ID (NCBI): 1266
• RRID: AB_2082140
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Cell Rep; Spatiotemporal dynamics of inner ear sensory and non-sensory cells revealed by single-cell transcriptomics.; Authors - Taha A Jan
human	WB	J Proteome Res; Comparative Proteomics Reveals Prolonged Corneal Preservation Impaired Ocular Surface Immunity Accompanied by Fibrosis in Human Stroma; Authors - Pei-Shan Wu
mouse	IHC	Mol Cell Biochem; STAT3 mediates ECM stiffness-dependent progression in ovarian cancer; Authors - Sun Chenchen
human	WB	Gastroenterol Res Pract; PMID: 30911294; Authors - Keun-Seok Hong ; KD Validated