CNN2 Polyclonal antibody

• Featured Product
• KD/KO Validated

CNN2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 21073-1-AP

Synonyms

calponin 2, Calponin H2, smooth muscle, CNN2, Neutral calponin

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Validation Data Gallery

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  WB analysis of HeLa using 21073-1-AP

  WB result of CNN2 antibody (21073-1-AP; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNN2 transfected HeLa cells.

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  WB analysis using 21073-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21073-1-AP (CNN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 21073-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21073-1-AP (CNN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 21073-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21073-1-AP (CNN2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human hysteromyoma using 21073-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 21073-1-AP (CNN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hysteromyoma using 21073-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 21073-1-AP (CNN2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 21073-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CNN2 antibody (21073-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 21073-1-AP

  Immunofluorescent analysis of HepG2 cells, using CNN2 antibody 21073-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IHC detected in: human heart tissue, human hysteromyoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

21073-1-AP targets CNN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CNN2 fusion protein Ag15225
• Full Name: calponin 2
• Calculated Molecular Weight: 309 aa, 34 kDa
• Observed Molecular Weight: 34-36 kDa
• GenBank Accession Number: BC141818
• Gene Symbol: CNN2
• Gene ID (NCBI): 1265
• RRID: AB_10695503
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). Calponin 1 and calponin 2 are predominately expressed in smooth muscle cells and cardiac muscle cells, respectively. Calponin 3 is highly expressed in many tissues including articular cartilage and brain.

Publications

Species	Application	Title
mouse	IF	Circ Res; Mitochondrial Protein Poldip2 Controls VSMC Differentiated Phenotype by O-Linked GlcNAc Transferase-Dependent Inhibition of a Ubiquitin Proteasome System.; Authors - Felipe Paredes
human	IHC, WB	iScience; Inhibitor of Differentiation 4 (ID4) represses mammary myoepithelial differentiation via inhibition of HEB.; Authors - Holly Holliday
rat	WB	Front Pharmacol; Activation of the Cholinergic Anti-inflammatory Pathway Attenuated Angiotension II-Dependent Hypertension and Renal Injury.; Authors - Shu-Jie Wu
human	WB	Tumour Biol; Knockdown of calponin 2 suppressed cell growth in gastric cancer cells.; Authors - Jianwei Hu ; KD Validated
mouse	IF	Elife; Differential regulation of hair cell actin cytoskeleton mediated by SRF and MRTFB; Authors - Ling-Yun Zhou
mouse,rat	WB,IHC	Mol Metab; Calponin 2 harnesses metabolic reprogramming to determine kidney fibrosis; Authors - Yuan Gui ; KD Validated