CNKSR2 Polyclonal antibody

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  WB analysis using 24054-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24054-1-AP (CNKSR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CNKSR2 Polyclonal Antibody for WB, ELISA

Cat No. 24054-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

CNK homolog protein 2, CNK2, CNKSR2, Connector enhancer of KSR 2, KIAA0902, KSR2

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24054-1-AP targets CNKSR2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CNKSR2 fusion protein Ag21183
• Full Name: connector enhancer of kinase suppressor of Ras 2
• Calculated Molecular Weight: 1034 aa, 118 kDa
• Observed Molecular Weight: 110 kDa, 130 kDa
• GenBank Accession Number: BC126121
• Gene Symbol: CNKSR2
• Gene ID (NCBI): 22866
• RRID: AB_3669430
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q8WXI2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Connector Enhancer of Kinase Suppressor of Ras-2 (CNKSR2), also known as CNK2 or MAGUIN, KIAA0902 and KSR2, is a scaffolding molecule that contains functional protein binding domains (PMID: 35053419). CNKSR2 interacts with different molecules, including RAF1, ARHGAP39, and CYTH2, and regulates the Mitogen-Activated Protein Kinase (MAPK) cascade and small GTPase signaling (PMID: 35053419). The study identify a molecular complex comprising CNKSR2 and the guanine nucleotide exchange factor (GEF) for ARF small GTPases, CYTH2, that is necessary for the proper development of granule neurons in the mouse hippocampus (PMID: 34800437). CNKSR2 plays a key role in regulating cell proliferation, and differentiation through multiple receptor tyrosine kinase pathways (PMID: 35053419). The molecular weight of CNKSR2 is mainly 117 kDa (PMID: 29534682). It has also been reported that CNKSR2 immunoblotted signal of about 110 kDa was detected, while a 130 kDa signal was detected (PMID: 34800437).