CNBP Polyclonal antibody

• Featured Product
• KD/KO Validated

CNBP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA, IF

Conjugate

Unconjugated

Publications

4

Cat no : 14717-1-AP

Synonyms

RNF163, PROMM, DM2, CNBP1, Cellular nucleic acid-binding protein

---

Validation Data Gallery

• 

  WB analysis of Caco-2 using 14717-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 14717-1-AP (CNBP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14717-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14717-1-AP (CNBP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14717-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14717-1-AP (CNBP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 14717-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14717-1-AP (CNBP Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 14717-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14717-1-AP (CNBP Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  IHC staining of human ovary using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: Caco-2 cells, HeLa cells, mouse heart tissue, mouse testis tissue, U-251 cells
• Positive IHC detected in: human ovary tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human skin tissue, human spleen tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 2 publications below

Product Information

14717-1-AP targets CNBP in WB, IHC, ELISA, IF applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CNBP fusion protein Ag6441
• Full Name: CCHC-type zinc finger, nucleic acid binding protein
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 20-25 kDa
• GenBank Accession Number: BC000288
• Gene Symbol: CNBP
• Gene ID (NCBI): 7555
• RRID: AB_2081548
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Single stranded DNA-binding protein, with specificity to the sterol regulatory element (SRE). Involved in sterol-mediated repression.

Publications

Species	Application	Title
human	WB	PLoS Biol; Phosphoglucomutase 1 inhibits hepatocellular carcinoma progression by regulating glucose trafficking.; Authors - Guang-Zhi Jin ; KD Validated
mouse	WB	Mol Cell Proteomics; Quantitative proteomics reveals the essential roles of STIM1 in the testicular cord formation in mouse testis.; Authors - Bo Zheng
mouse	WB,IF	iScience; Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis.; Authors - Qianxing Zou
mouse	WB,IF	Reproduction; Cellular nucleic acid-binding protein is vital to testis development and spermatogenesis in mice.; Authors - Bo Zheng